DNA Extraction from Paraffin Embedded Material

 

This video demonstrates the protocol for DNA extraction from formalin-fixed paraffin-embedded material. This is a multi-day procedure in which tissue sections are deparaffinized with xylene, rehydrated with ethanol and treated with proteinase K to purify and isolate DNA for subsequent gene-specific or genome-wide analysis.

 

 

Authors:
Larissa A. Pikor, Katey S. S. Enfield, Heryet Cameron, Wan L. Lam